Objective To construct a melanoma cell strain expressing endotopic CSDE1-EGFP using new CRISPR-PITCH system. then to select the cell strain of low expression CSDE1 and high expression CSDE1. Methods The EGFP coding sequence was inserted into the last exon of the CSDE1 gene using a micro-homology-mediated end joining(MMEJ) method; Using flow cytometry sorting. PCR. https://www.pomyslnaszycie.com/chocolate-vibrating-table-on-sale/