: Figure out the cycle at which the next by-product of fluorescence with regard to cycle selection is maximal (Rasmussen, 2001). Local fitting into a parabola may very well be used to define the peak situation with sub‐cycle precision. T7 RNA polymerase from other suppliers may very well be used, https://www.facebook.com/NewTechFind/posts/pfbid0WAXHtwnNeSeF5c4UfH6rpToKz3Pi5u11Dphz4ANFrDecmNto52vA4cw8qJtmxoDdl