The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR products were digested and cloned right into a pET21a+ vector making use of NdeI and XhoI restriction enzymes. A point mutation was launched by the https://juliac107ppn3.salesmanwiki.com/user